Part of #The conformational changes of DNA by mobile phone radiofrequency (940 MHz)# :
Publishing year : 2012
Conference : The First International Conference on Iran's Environmental Crisis and its Improvement
Number of pages : 9
Abstract: Currently, the biological effects of nonionizing electromagnetic fields (EMFs), including radiofrequency (RF) radiation, have been subject to numerous experimental and theoretical studies. The purpose of this study is to evaluate the possible biological effects of mobile phone RF (940 MHz, 15 V / m and SAR = 40 mW / kg) on the DNA structure immediately after exposure and 2 h after exposure to 45 min across a wide range Of spectroscopic instruments. The UV-Visible and CD experiments depict that mobile phone EMFs can remarkably cause disturbance on the DNA structure. In addition, the DNA samples, immediately after exposure and 2 h after 45 min exposure, are relatively thermally unstable compared to the DNA solution, which was placed in a small shielded box (unexposed DNA). Furthermore, the exposed DNA samples (the DNA samples that were exposed to the 940 MHz EMF) have more fluorescence emissions when compared to the unexposed DNA, which may have been attributed to the expansion of the exposed DNA structure. The results of dynamic light scattering (DLS) and zeta potential experiments show that RF-EMFs lead to increment in the surface charge and size of DNA. The structure of DNA immediately after exposure is not significantly different from the DNA sample 2 h after exposure to 45 min. In other words, the EMF-induced conformational changes are irreversible. Collectively, our results show that 940 MHz can alter the structure of DNA. The displacement of electrons in DNA by EMFs may lead to conformational changes in DNA and DNA disaggregation. Results from this study could have a significant impact on the health effects of exposure to RF-EMFs.