Covalent attachment of acetylcholinesterase on electrospun poly vinyl alcohol nanofiber:potential use in pesticide biosensor

Covalent attachment of acetylcholinesterase on electrospun poly vinyl alcohol nanofiber:potential use in pesticide biosensor

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Part of #Covalent attachment of acetylcholinesterase on electrospun poly vinyl alcohol nanofiber:potential use in pesticide biosensor# :

Publishing year : 2008

Conference : The 2nd International Congress on Nanosciences and Technology

Number of pages : 5

Abstract: Poly vinyl alcohol (PVA) nanofibers can be fabricated by electrospinning, and provide a large surface area for enzyme immobilization and catalytic reactions. In this study, acetylcholinesterase from Drosophila melanogaster (AChE) (EC 3.1.1.7) has been successfully immobilized on poly vinyl alcohol (PVA) nanofibers by electrospinning of a mixture of acetylcholinesterase, bovine serum albumin (BSA) as an enzyme stabilizing additive and poly vinyl alcohol (PVA). The surface of nanofibrous membranes were covalently bonded to BSA and AChE via glutaraldehyde vapors. Immobilized enzyme activity was assayed by spectrophotometric technique and showed that immobilized AChE retained 40% of the free enzyme activity. The kinetic parameters of the free and immobilized AChE, Km and Kcat were also determined. Moreover, the enzyme nanofiber was found to be very stable, since the immobilized AChE retained & gt; 70% of its initial activity when stored in a buffer at 25 C for 53 days and 90% at 4 C for 60 days. immobilized enzymes retained 90% of its initial activity after 10 consecutive reactor batch cycles. This compartment could be used for biosensor design for pesticide determination