Part of #Enhanced Plantlet Regeneration from Cultured Meristems in Sprouting Buds of Saffron Corms# :
Publishing year : 2006
Conference : Second International Symposium on Technology and Biology of Saffron
Number of pages : 5
Abstract: When meristems were dissected from dormant corms were used for micropropagation of saffron, low and heterogeneous morphogenic potential as well as high contamination levels were found (Piqueras and Hernandez, 2004). With the aim of preventing the above mentioned inconvenient, shoot cultures have been initiated from meristems in sprouted saffron corms before flowering. These experiments showed a significant lower contamination rate (less than 30%) when cultured in culture medium, composed of QL salts, MS vitamins and 30 g / l sucrose, solidified with 7.5 g / l agar. To study the morphogenic response induced by different cytokinin types and concentrations (BAP, 2iP and TDZ), the explants were cultivated in medium supplemented with them for sex weeks. After this period, the number of new shoots per initial explant, their length and quality were recorded and used as parameters for selecting the most effective cytokinin level for multiplication. Using this new explant for initiation of axillary shoot cultures in a saffron, a new micropropagation procedure has been developed for the selection of selected genotypes for clonal propagation or ex situ germplasm conservation.