Part of #Immunization of sheep with whole body of Echinococcus granulosus# :
Publishing year : 2005
Conference : Fourth National Biotechnology Congress of Iran
Number of pages : 8
Abstract: In this study, two hundred mature Echinococcus granulosus, which were kept in 10% formalin for 8 months, obtained from Parasitology Depatrment, School of Veterniary Medicine, Ferdowsi University of Mashhad. Protein soluble parasite was prepared. The sample is hemogenized in a blender, sonicated on ice and then centrifuged for 15 min at 10000 g. Final yield was kept at -20 C until used. Eight Lambs 4 to 6 months of age of mixed sex were divided into 2 groups of 4 lambs. Each lamb in the test group was vaccinated subcutaneously in the neck with a 2 ml dose of vaccine [1 mg of whole body of E. granulosus protein, dissolved in 1 ml of PBS plus 1 ml of Freund's complete adjuvant (FCA)]. Control lambs were vaccinated with adjuvant in PBS. Lambs were re-vaccinated four weeks after the first vaccination with the same preparation, except that FCA was replaced by Freund's incomplete adjuvant (FIA). Three weeks after the second vaccination, each lamb received a challenge infection with 2000 protoscolices intrapritoneally and 10 adult E. granulosus. All sheep were killed after 7 months and examined for hydatid cysts. Results showed that, in comparison with control lambs, in one of the vaccinated lamb two and one cyst were observed in the liver and liver, respectively. The number of cysts in vaccinated lambs was significantly lower than in the control group (P & lt; 0.001). This result indicates that the protective immunity in sheep with the whole body of E. granulosus was approximately 90%.